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KMID : 0545119930030030156
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Journal of Microbiology and Biotechnology
1993 Volume.3 No. 3 p.156 ~ p.160
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Expression of Chimeric Chicken-Yeast-Chicken H2B Histone Gene
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Son Seung-Yeol
Lee Ho
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Abstract
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A chicken H2B histone gene was cloned and expressed in Rat 3 cell line. Its messenger RNA level was about 10 times higher during S phase than during G_1 phase. A chimeric chicken-yeast-chicken H2B histone gene was made to change some of wobble sequences of chicken H2B gene. When the chimeric H2B gene was transfected into the Rat 3 cell line, it showed a pattern of expression similar to that of the original chicken H2B gene. At least in this gene, it was concluded that the wobble sequences were not required for the cell-cycle regulated pattern of expression.
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